Laboratory Techniques

TaqMan real-time PCR assay is sensitive, reliable for quantitating HCV RNA

Published in Medical Letter on the CDC and FDA, December 29th, 2002

U.S. Food and Drug Administration (FDA) researchers have developed what they say is a highly reproducible, sensitive, and reliable assay for hepatitis C virus RNA quantitation using the TaqMan PCR (polymerase chain reaction) system.

"The availability of molecular protocols for the detection and quantitation of very low numbers of hepatitis C virus (HCV) particles in biological samples is an issue of interest in both clinical and analytical fields of HCV research. A sensitive and reproducible assay is described for HCV RNA quantitation using the TaqMan PCR fluorogenic real-time detection system to establish the levels of HCV RNA in chimpanzee plasma," wrote M. Puig and...

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Source: Medical Letter on the CDC and FDA (2002-12-29)

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