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Food-borne Illness

Microplate sandwich assay suitable for detection of E. coli O157:H7 in food

Published in Medical Letter on the CDC and FDA, December 28th, 2003

A novel microplate sandwich immunoassay is suitable for detection of E. coli O157:H7 contamination in food.

According to published research from the United States, "Escherichia coli O157:H7 has been identified as a causative agent in outbreaks of hemorrhagic colitis and hemolytic uremic syndrome involving ground beef and apple cider."

"A microplate sandwich immunoassay for the detection of E. coli O157:H7 from food was developed utilizing time-resolved fluorometry," reported L.S.L. Yu and coauthors at the U.S. Department of Agriculture.

"Biotin-labeled, polyclonal anti-E. coli O157:H7 antibodies...

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