Botulinum Toxin

Mouse bioassay and ELISA compared for detecting botulinum toxin in food

Published in Medical Letter on the CDC and FDA, March 14th, 2004

Researchers have compared the mouse bioassay and enzyme-linked immunosorbent assay procedures for the detection of type A botulinal toxin in food.

"Samples of chili linked to a food-borne illness outbreak of type A botulism were examined for preformed type A botulinal toxin using two enzyme-linked immunosorbent assay (ELISA) procedures and the mouse bioassay. One of the samples was positive for type A botulinal toxin and three of the samples were negative for type A, B, E, and F botulinal toxins using the three methods. The mouse bioassay indicated that type A toxin was present at the 10,000 minimal lethal dose per gram (MLD per g) of product," scientists writing in the...

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Source: Medical Letter on the CDC and FDA (2004-03-14)

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