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Anthrax

New assay better than ELISA for measuring antibodies to anthrax toxins

Published in Medical Letter on the CDC and FDA, March 21st, 2004

A fluorescence covalent microbead immunosorbent assay (FCMIA) was more rapid and efficient than an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins.

"Recently, the Centers for Disease Control and Prevention reported an accurate, sensitive, specific, reproducible, and quantitative enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis protective antigen (PA) in human serum. The ELISA had a minimum detectable concentration (MDC) of 0.06 microgram/mL, which, when dilution adjusted, yielded a whole-serum MDC of 3.0 micrograms of anti-PA IgG per mL," scientists in...

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