Vector Development
Expression of exogenous genes in Trypanosoma cruzi improved
Published in Medical Letter on the CDC and FDA, April 4th, 2004
"To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient transfections, the electroporation conditions reported here resulted in luciferase expression 100 times higher than the levels obtained with previously described protocols," scientists in Brazil and Argentina report.
"To verify whether sequences containing different trans-splicing signals influence reporter gene expression, we compared DNA...
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