Chagas Disease
Basis of non-virulence of Trypanosoma cruzi clone determined
Published in Medical Letter on the CDC and FDA, August 29th, 2004
"We investigated the properties of metacyclic trypomastigotes of non-virulent Trypanosoma cruzi clone CL-14, as compared to the parental isolate CL. In contrast to the CL isolate, which produces high parasitemias in mice, metacyclic forms of clone CL-14 failed to produce patent infection. In vitro, the number of clone CL-14 parasites that entered epithelial HeLa cells, after 1-hour incubation, was approximately four-fold lower than that of the CL isolate and at 72 hours post-infection intracellular replication was not apparent whereas cells infected with...
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