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Stem Cell Research

Serum-free media improve generation of embryonic stem cells

Published in Medical Letter on the CDC and FDA, September 26th, 2004

Serum-free media improve the generation of embryonic stem cells.

"C57BL/6 is a well-characterized mouse strain that is used extensively for immunological and neurological research. The establishment of C57BL/6 ES cell lines has facilitated the study of gene-altered mice in a pure genetic background; however, relatively few such lines exist," investigators in the United States report.

"Using a defined media supplement, knockout serum replacement (KSR) with knockout DMEM (KSR-KD- MEM)," said J. Cheng and colleagues, "we find that we can readily establish ES cell lines from blastocysts of C57BL/6J mice."

"Six lines were established,...

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