Efficacy of diluted smallpox vaccine remains high when stored on ice

According to recent research from the United States, "During a mass smallpox immunization campaign, vaccine may be exposed to ambient temperatures for extended periods of time. To determine the viability of undiluted and 5 x diluted DryVax smallpox vaccine after cycling vaccine in and out of refrigeration for two weeks, as might occur during an immunization campaign. Two vials of Dryvax vaccine were reconstituted as per manufacturer's instructions (1 x) and two vials were reconstituted using 5 x the recommended diluent (5 x)."

"Every 12 hours over two weeks, vials were cycled...

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