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Dengue Virus

Fourplex real-time RT-PCR assay to detect dengue viruses is developed

Published in Medical Letter on the CDC and FDA, December 25th, 2005

Researchers have developed a method for the serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay.

"The dengue (DEN) viruses are positive-strand RNA viruses in the genus Flavivirus. Dengue fever and dengue hemorrhagic fever/dengue shock syndrome are important human arboviral diseases caused by infection with one of four closely related but serologically distinct DEN viruses, designated DEN-1, DEN-2, DEN-3, and DEN-4 viruses. All four DEN serotypes are currently co-circulating throughout the subtropics and tropics, and genotypic variation occurs among isolates within a serotype," scientists...

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