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Proteomics

2-D protein maps of rat gastrocnemius and soleus muscles constructed

Published in Medical Letter on the CDC and FDA, March 5th, 2006

Protein maps of the gastrocnemius and soleus muscles have been constructed using 2-dimensional gel electrophoresis (2-DE) and mass spectrometry.

"Functional characterization of muscle fibers relies on ATPase activity and on differential measurements of metabolic proteins, including mitochondrial and glycolytic enzymes, glucose, lactate and lactic acid transporters, calcium cycling proteins and components of the contractile machinery," scientists in Italy explained.

"The recent introduction of microarray technology has enabled detailed gene expression studies under different physiological and pathological conditions, thus generating novel hypotheses...

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