Polymerase Chain Reaction
PCR method to detect toxin genes in enterotoxigenic Escherichia coli has been developed
Published in Medical Letter on the CDC and FDA, April 26th, 2006
Scientists in the United States report, "A multiplex real-time PCR method was developed for detection of heat-labile and heat-stable toxin genes in enterotoxigenic Escherichia coli. Approximately 10 CFU per reaction mixture could be detected in rinsates from produce samples. Several foods representative of varieties previously shown to have caused enterotoxigenic E. coli outbreaks were spiked and enriched for four or six hours."
"Both heat-labile and heat-stable toxin genes...
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