Enzyme Research
Investigators at Biomed Center target enzyme research
Published in Medical Letter on the CDC and FDA, November 9th, 2008
"Thymidylate kinase is an ideal target since this enzyme is at the junction of the de novo and salvage synthesis of dTTP, an essential precursor for DNA synthesis. Here the expression and characterization of thymidylate kinase from B. anthracis (Ba-TMPK) is presented. The enzyme phosphorylated deoxythymidine-5'-monophosphate (dTMP) efficiently with K-m and V-max values of 33 mc M and 48 mu mol mg(-1) min(-1), respectively. The efficiency of...
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