Second Military Medical College, Shanghai
Residual host cell DNA in plasmid DNA is quantitated by gene probe
Published in Medicine and Law Weekly, May 19th, 2006
"The development and widespread use of DNA-based vaccination against infectious pathogens have been a great triumph of medical science. Quality control of DNA vaccines as biopharmaceutical productions is a problem to solve. Residual genomic DNA of engineering bacteria has been identified as a potential risk factor, so whose level must be controlled under the regulatory standards," scientists in China note.
"We report a dot-blot hybridization method to detect residual host cell DNA in purified DNA vaccines," announced Kai-Yu Wang and colleagues at the Second...
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