Chemical modification of insulin occurs in amyloid fibrils

"We have investigated the chemical modification of insulin under conditions that promote the conversion of the soluble protein into amyloid fibrils. The modifications that are incorporated into the fibrils include deamidation of Asn A21, Asn B3, and Gln B4. In order to prepare fibrils with minimal deamidation of these residues, the kinetics of aggregation were accelerated by seeding with aliquots of a solution containing preformed fibrils," investigators in England report.

"The resulting fibrils were then re-incubated to determine the extent to which chemical modification occurs in the...

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