Adipogenesis
Flow cytometric method used to assess lipid accumulation in fat cells
Published in Obesity and Diabetes Week, August 2nd, 2004
"Adipogenesis of preadipocytes in culture has been frequently used to study the molecular basis and effect of drugs on fat cell conversion. However, after adipogenic induction, cells respond to the inducing agent with various speeds of conversion and fat accumulation, which complicates direct molecular and biochemical analyses. Here we present a simple and sensitive method to detect and quantify fat accumulation inside cells by flow cytometry," researchers in Taiwan and Germany report.
"Using this method, we detected elevated levels of cytoplasmic...
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