U.S. Food & Drug Administration
Azoreductase from Staphylococcus aureus characterized
Published in Pharma Investments, Ventures and Law Weekly, July 17th, 2005
According to recent research from the United States, "Azo dyes are a predominant class of colorants used in tattooing, cosmetics, foods, and consumer products. A gene encoding NADPH-flavin azoreductase (Azol) from the skin bacterium Staphylococcus aureus ATCC 25923 was identified and overexpressed in Escherichia coli. RT-PCR results demonstrated that the azol gene was constitutively expressed at the mRNA level in S. aureus. Azol was found to be a tetramer with a native molecular mass of 85 kDa containing four non-covalently bound FMN. Azol requires NADPH, but not NADH,...
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