Clostridium
New clostridium study findings have been published by scientists at Kagawa University
Published in Pharma Law Weekly, October 21st, 2008
According to recent research from Kagawa, Japan, "Clostridium histolyticum collagenase is used to isolate cells from various organs and tissues for tissue engineering, and also to treat destructive fibrosis; thus, the demand for high-grade enzyme preparations is increasing. In this study, we constructed a plasmid encoding C. histolyticum type II collagenase (ColH) with a C-terminal hexahistidine tag (ColH-his) to facilitate the purification of the enzyme through immobilized metal affinity chromatography (IMAC)."
"When ColH-his was expressed in a protease-deficient mutant of Clostridium perfringens, it was produced in the culture supernatant more efficiently than the...
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Source: Pharma Law Weekly (2008-10-21)
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