Univ. of California, San Francisco
Methods to recover cells from ELISPOT immunoassay developed
Published in Physician Law Weekly, September 1st, 2004
"The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive and reproducible method for quantifying T cell-mediated immune responses, and has been used to measure antigen-specific responses post-vaccination. While there are several advantages of the ELISPOT assay for use in field settings for large-scale vaccination trials, blood draw volumes are often limited, and the number of antigen-specific responses that can be measured is constrained by the limited cell number. We reasoned that it should be possible to salvage and rescue viable cells from a completed ELISPOT assay...
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Source: Physician Law Weekly (2004-09-01)
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