University of Nebraska

Antigenicity of HIV-1 p24 produced in high yields from plants was preserved

Published in Physician Law Weekly, December 15th, 2004

The antigenicity of HIV-1 p24 produced in high yields from plants inoculated with a tobacco mosaic virus -derived vector was preserved.

"Production of structural proteins from foot-and-mouth disease virus (FMDV) and bovine herpes virus (BHV-1) in Nicotiana benthamiana through the use of a tobacco mosaic virus-based vector (TMV-30B) has been reported previously. The development of the TMV-30B-HISc vector, a new version that adds a C-terminal histidine (His) sequence to the foreign protein expressed is described. Coding sequences from the FMDV VP1 protein and the core protein, p24, from a clade C HIV-1 isolate from Zambia were cloned into the new vector and...

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Source: Physician Law Weekly (2004-12-15)

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