Physician Law Weekly
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All India Inst. of Medical Science, New Delhi
Reporter replicons establish a way to analyze hepatitis E virus biology
June 15th, 2005
Reporter replicons establish a way to analyze hepatitis E virus biology. "Hepatitis E virus (HEV) replication has been demonstrated in HepG2 cells transfected with full-length in vitro transcripts of an infectious cDNA clone. This cDNA clone was modified to generate several subgenomic HEV replicons with fused reporter genes," scientists in India report. "In vitro-transcribed capped RNAs generated from these were transfected into HepG2 cells. Negative-strand RNA was detected, indicating the occurrence of replication. The replicon containing an in-frame fusion of HEV ORF2 with enhanced green fluorescent protein (EGFP) was positive for fluorescence,...
Source: Physician Law Weekly (2005-06-15)
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