Osteoarthritis
Collagen synthesis, regulatory molecule expression studied in osteoarthritis
Published in Proteomics Weekly, December 20th, 2004
"We show that proteomic analysis can be applied to study cartilage pathophysiology. Proteins secreted by articular cartilage were analyzed by two-dimensional SDS-PAGE and mass spectrometry. Cartilage explants were cultured in medium containing [S-35] methionine/cysteine to radiolabel newly synthesized proteins."
"To resolve the cartilage proteins by two-dimensional electrophoresis, it was necessary to remove the proteoglycan aggrecan by precipitation with cetylpyridinium chloride."
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Source: Proteomics Weekly (2004-12-20)
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