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Chromatography

Model predicts retention of proteins during FPLC

Published in Proteomics Weekly, May 23rd, 2005

Researchers have applied the stoichiometric displacement model to describe the retention behavior of charged-fusion proteins during fast protein liquid chromatography (FPLC).

According to a study from the United States, "The applicability of the stoichiometric displacement model (SDM) to description of the retention behavior of charged-fusion proteins in large ion exchange resin ({{approx}}90 micrometer diameter) packed column was studied. Proteins were characterized by SDM for isocratic elution. The parameters were subsequently used to evaluate their suitability in predicting protein retention and peak width under gradient elution. The proteins were beta-glucuronidase...

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