Published in Proteomics Weekly, August 21st, 2006
"The plasmid pET-21d-2c-5B Delta 55 effectively expressing a C-terminally truncated form (NS5B Delta 55) of the HCV RNA-dependent RNA polymerase (RdRp) was constructed. It was derived from pET-21d-5B Delta 55 plasmid and contained six mutations in the ATG-start codon region and an additional cistron upstream the target gene."
"The C-terminally His-tagged NS5B Delta 55 protein was expressed in Rosetta(DE3) Escherichia coli strain bearing an additional pRARE plasmid encoding...
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