Fusion Proteins

Study results from Yonsei University, Department of Anatomy provide new insights into fusion proteins

Published in Proteomics Weekly, January 1st, 2007

A report, "Efficient cellular uptake of recombinant murine Hoxc8 homeoprotein in COS-7 cells," is newly published data in Life Sciences. "In order to analyze the self-delivery activity of Hoxc8, recombinant Hoxc8 protein (rHoxc8) was designed to be expressed and purified in E. coli as a glutathione S-transferase and green fluorescent protein-fused form (GST-GFP-Hoxc8)," scientists in Seoul, Korea report.

"After purification using glutathione sepharose beads, the 82 kDa fusion protein was separated on the SDS-PAGE gel and confirmed by detecting the fluorescence through luminescent image analyzer. When rHoxc8 was added to culture media for 30 h, most of the COS-7...

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Source: Proteomics Weekly (2007-01-01)

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