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Enzyme Research

Reports outline enzyme research from Shizuoka University

Published in Proteomics Weekly, March 31st, 2008

According to a study from Shizuoka, Japan, "A bacmid system of BmMNPV [Bombyx mori (silkworm) multiple nucleopolyhedrovirus] with both the cysteine-protease and chitinase genes deleted (BmMNPV-CP--Chi(-)) was constructed."

"The viral protease and chitinase activities in the haemolymph of Bombyx mori larvae infected with this BmMNPV-CP--Chi(-) bacmid were reduced by 95 and 50% respectively. By using this system, a GFP(uv)-beta 3GnT2 (green fluorescent protein excited by UV light-beta 1,3-N-acetylglucosaminyltransferase 2) fusion protein was successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction; beta 3GnT2 activity...

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