Proteomics Weekly
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Gene Therapy
Research in the area of gene therapy reported from University of Chile
May 12th, 2008
"Antarctic seawater bacteria producing extracellular lipolytic enzymes with activity at low temperatures were isolated. The most promising strain was selected to perform a 16S characterization which identified it as a Psychrobacter sp," scientists in Santiago, Chile report. "The genomic DNA of this bacterium was used for a PCR screening using primers obtained from multiple sequence alignments of lipases belonging to the hormone sensitive lipase (HSL) group of the prokaryotic species. This allowed cloning and sequencing of the DNA that partially encodes a novel lipase protein (240 bp, 80 aa). Subsequently the complete gene was obtained by a genome-walking technique. An open...
Source: Proteomics Weekly (2008-05-12)
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