Gene Therapy

Research from K. Dohi and colleagues provide new insights into gene therapy

Published in Proteomics Weekly, November 10th, 2008

"A major obstacle in the genetic manipulation of tomato mosaic virus (ToMV) is the instability of the plasmid containing the infectious full-length cDNA of the ToMV vector, which often prevents the subcloning of a foreign gene of interest into the vector," researchers in Ishikawa, Japan report.

"We found that an insertion of a 0.3-1.6-kbp DNA fragment in the movement protein (MP) coding region effectively attenuated bacterial toxicity of the plasmid and greatly increased plasmid yield. Accumulation of a modified ToMV containing a 0.3-kb insertion in the MP coding region was comparable to that of a modified ToMV without an insertion in tobacco BY-2 protoplasts, while an...

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Source: Proteomics Weekly (2008-11-10)

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