Proteomics Weekly
Welcome to NewsRx!
Learn more about a six-week, no-risk free trial of Proteomics Weekly
We're a pay-per-view site for premium content. If you'd like to purchase this article, it's only $3.00.
Proteomics
Research results from University of Milan update understanding of proteomics
July 13th, 2009
According to a study from Milan, Italy, "We describe the characterization of polyclonal antibodies directed against the whole mitochondrial subproteome, as obtained by hyperimmunization of rabbits with an organelle fraction purified from human skeletal muscle and lysed by sonication. After 2-DE separations with either blue native electrophoresis or IPG as first dimension and blotting, the polyspecific antibodies detect 113 proteins in human muscle mitochondria, representative of all major biochemical pathways and oxidative phosphorylation (OXPHOS) complexes, and cross-react with 28 proteins in rat heart mitochondria." "Using as sample cryosections of human muscle...
Source: Proteomics Weekly (2009-07-13)
|
