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Proteomics Weekly

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Klebsiella pneumoniae



New Klebsiella pneumoniae data have been reported by scientists at Chonnam National University



July 20th, 2009

"An artificial fusion protein of Arthrobacter oxydans dextranase and Klebsiella pneumoniae alpha-amylase was constructed and expressed in Escherichia coli," scientists in South Korea report.

"Most of the expressed protein existed as an insoluble fraction, which was solubilized with urea. The purified fusion enzyme electrophoretically migrated as a single protein band; M = 137 kDa, and exhibited activities of both dextranase (10.8 U mg(-1)) and amylase (7.1 U mg(-1)), which were lower than that of reference dextranase (13.3 U mg(-1)) and alpha-amylase (103 U mg(-1))," wrote Y.M. Kim and colleagues, Chonnam National University.

The researchers...


Source: Proteomics Weekly (2009-07-20)

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