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Proteomics

Research conducted at Johann Wolfgang Goethe University has updated our knowledge about proteomics

Published in Proteomics Weekly, August 2nd, 2010

Researchers detail in 'Laser-induced liquid bead ion desorption-MS of protein complexes from blue-native gels, a sensitive top-down proteomic approach,' new data in proteomics. "We have developed an experimental approach that combines two powerful methods for proteomic analysis of large membrane protein complexes: blue native electrophoresis (BNE or BN-PAGE) and laser-induced liquid bead ion desorption (LILBID) MS. Protein complexes were separated by BNE and eluted from the gel," scientists in Frankfurt am Main, Germany report.

"The masses of the constituents of the multiprotein complexes were obtained by LILBID MS, a detergent-tolerant method that is especially...

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