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Enzymes and Coenzymes

Reports from Thomas Jefferson University advance knowledge in enzymes and coenzymes

Published in Proteomics Weekly, October 18th, 2010

Investigators publish new data in the report 'Distinct kinetic determinants for the stepwise CCA addition to tRNA.' "The universally conserved CCA sequence is present at the 3' terminal 74-76 positions of all active tRNA molecules as a functional tag to participate in ribosome protein synthesis. The CCA enzyme catalyzes CCA synthesis in three sequential steps of nucleotide addition at rapid and identical rates," scientists writing in the journal Rna report.

"However, the kinetic determinant of each addition is unknown, thus limiting the insights into the kinetic basis of CCA addition. Using our recently developed single turnover kinetics of Escherichia...

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