DNA Research
Findings from University of Southern California, Department of Chemistry in DNA research reported
Published in Proteomics Weekly, November 1st, 2010
A new study, 'A nucleotide-independent nitroxide probe reports on site-specific stereomeric environment in DNA,' is now available. According to a study from the United States, "In this report, stereospecific structural and dynamic features in DNA are studied using the site-directed spin labeling technique. A stable nitroxide radical, 1-oxyl-4-bromo-2,2,5,5-tetramethylpyrroline (R5a), was attached postsynthetically to phosphorothioates that were chemically introduced, one at a time, at five sites of a DNA duplex."
"The two phosphorothioate diastereomers (R(p) or S(p)) were separated, and nitroxide rotational motions were monitored using electron paramagnetic resonance...
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Source: Proteomics Weekly (2010-11-01)
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