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Embryonic Stem Cell Research
Embryonic stem cell electroporation enhances small and macromolecule loading efficiency
August 14th, 2006
Embryonic stem cell electroporation enhances small and macromolecule loading and DNA transfection efficiency. "Cryopreservation, directed differentiation, and genetic manipulation of human embryonic stem cells (hESCs) all require the transport of exogenous small molecules, proteins, or DNA into the cell. "The absence of standard small and macromolecule loading techniques in hESCs as well as the inadequacies of current DNA transfection techniques have led us to develop electroporation as an efficient loading and transfection methodology," investigators in the United States report. According to J.C. Mohr and colleagues at the...
Source: Stem Cell Week (2006-08-14)
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