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Stem Cell Research
Stem cell factor fused with mimetic peptide in Escherichia coli
August 14th, 2006
Recent research from the People's Republic of China has detailed the cloning and expression of human stem cell factor (SCF) fused with thrombopoietin (TPO) mimetic peptide in Escherichia coli. "TPO acts synergistically with SCF in hematopoiesis and megakaryopoiesis. In this work, we designed the expression of SCF fused with the monomer or the dimer of TPO mimetic peptide through a flexible peptide linker. "The recombinant fusion proteins were produced in E. coli DH5 alpha at up to 25% of total cell proteins. The resultant inclusion bodies were refolded by dilution and purified by ion-exchange chromatography," wrote L. Su and colleagues,...
Source: Stem Cell Week (2006-08-14)
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