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Gene Therapy

Reports outline gene therapy research from Taipei Veterans General Hospital

Published in Stem Cell Week, June 25th, 2007

Researchers detail in "Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method," new data in gene therapy. Researchers in Taipei, People's Republic of China conducted a study "To enhance the differentiation of insulin producing cell (IPC) ability from embryonic stem (ES) cells in vitro. Four-day embryoid body (EB)-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery."

"The use of Nucleofector electroporator (Amaxa biosystems, Germany) in combination with medium-contained G418 provided a high efficiency of gene delivery for advanced selection. Neucleofected cells were plated on...

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