"Inactivation of Polymerase Inhibitors for Mycobacterium tuberculosis DNA Amplification in Sputum by using Capture Resin."

According to the authors' abstract of an article published in Journal of Clinical Microbiology, "Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P>0.05) and 100% (P-C and GR combined; P<0.05)." The corresponding author for this study is: C Saltini, Univ Modena, Dipartimento Sci Med Oncol & Radiol, Via Pozzo 71, I-41100 Modena, Italy. For subscription information for this journal contact the publisher: Amer Soc...

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