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"Algorithm for Use of Nucleic Acid Probes for Identifying Mycobacterium tuberculosis from BACTEC 12B Bottles."

Published in TB and Outbreaks Week, August 7th, 1995

According to the authors' abstract of an article published in Journal of Clinical Microbiology, "Nucleic acid probes (Gen-Probe, San Diego, Calif.) can be used to identify mycobacteria in BACTEC 12B broth cultures prior to detection of growth on solid media. We developed an algorithm that can be used to make an initial choice of a probe (either Mycobacterium tuberculosis complex [MTB] or M. avium complex [MAC]) for use in testing respiratory specimens. The algorithm was based on both the fluorochrome smear result of the concentrated specimen and the time from inoculation until the BACTEC 12B broth culture is flagged (growth index 10) as presumptively positive. The MTB...

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