Diagnostics (TB)

PCR-ELISA Enables Identification of Mycobacteria Within Clinical Specimens

Published in TB and Outbreaks Week, October 6th, 1997

A polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay (ELISA) has been developed which may be suitable for the detection and concurrent identification of mycobacteria within clinical specimens.

Currently, the most rapid and cost-effective method for diagnosing mycobacterial infection is microscopic examination of stained specimens. This method, however, lacks sensitivity and cannot always permit conclusive identification of the species present. Culture of the specimen and biochemical analysis of any isolate is slow, often taking many weeks.

Rapid genotypic methods have been investigated, resulting in the development of commercial...

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Source: TB and Outbreaks Week (1997-10-06)

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