Legionella
"Comparative Analysis of Infrequent-Restriction-Site PCR and Pulsed-Field Gel Electrophoresis for Epidemiological Typing of Legionella pneumophila Serogroup 1 Strains."
Published in TB and Outbreaks Week, March 9th, 1998
According to the authors' abstract of an article published in Journal of Clinical Microbiology, "Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup I isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of...
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