"A Parallel Evaluation of a Redox Dye (Alamar Blue)-Based, Colorimetric Broth Microdilution Assay with a Bactec-Based, Radiometric Broth Macrodilution Assay for Susceptibility Testing

According to an abstract submitted by the authors to the 37th ICAAC, held September 28 - October 1, 1997, in Toronto, Ontario, Canada, "There is no standard method of susceptibility testing of MAC isolates, although many laboratories use the Bactec 460 culture system. Bactec culture has several important limitations: (1) the Bactec machine has only a moderate throughput (60 vials/90 min.); (2) Bactec involves the use of radioactive materials; (3) comparatively large amounts of drug are required for testing; and (4) it is expensive. The aim of this study was to compare an alternative broth microdilution assay in a 96-well microtiter plate format with the Bactec T100 assay for the...

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