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Testing (TB)

In-House Testing Distinguishes M. tb from M. avium

Published in TB and Outbreaks Week, October 5th, 1998

Polymerase chain reaction amplification of the 16S-23S rRNA gene spacer appears to be a useful alternative in-house method for differentiating between Mycobacterium tuberculosis and M. avium.

The identification of mycobacterial species is usually done by time-consuming biochemical testing or with Accuprobe, which is expensive. Several other molecular genetic methods have also been reported, including amplification of species-specific sequences, polymerase chain reaction (PCR) amplification and restriction enzyme analysis (PCR-REA), hybridization with species-specific oligonucleotide probes with or without prior DNA amplification, and nucleic acid sequence...

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