Newcastle Disease Virus

Researchers Rate New Method of Detection

Published in TB and Outbreaks Week, May 23rd, 2000

In a recent study from Malaysia, researchers reported a sensitive and specific reverse-transcriptase-nested polymerase chain reaction (PCR) coupled with an enzyme-linked immunosorbent assay (ELISA) detection system may be useful in the screening of samples for Newcastle disease virus (NDV).

C.L. Kho and colleagues from the University of Putra Malaysia designed two nested pairs of primer that were highly specific to all the three different pathotypes of NDV. They did not observe any cross-reactions with other avian infectious agents including infectious bronchitis virus, infectious bursal disease virus, influenza virus, and fowl pox virus ("Performance of an RT-nested...

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Source: TB and Outbreaks Week (2000-05-23)

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