Infectious Disease

Direct random insertion mutagenesis effective in Helicobacter pylori

Published in TB and Outbreaks Week, March 4th, 2003

Researchers in the Netherlands have improved the process of random insertion mutagenesis, "a widely used technique for the identification of bacterial virulence genes."

"Most strategies for random mutagenesis involve cloning in Escherichia coli for passage of plasmids or for phenotypic selection," explained R. de Jonge and coauthors at Erasmus University. However, this method "can result in biased selection due to restriction or instability of the cloned DNA, or toxicity of the encoded products."

To remedy this, de Jonge and coworkers "created two mutant libraries in the human pathogen Helicobacter pylori using a simple, direct...

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Source: TB and Outbreaks Week (2003-03-04)

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