Published in TB and Outbreaks Week, March 4th, 2003
"Most strategies for random mutagenesis involve cloning in Escherichia coli for passage of plasmids or for phenotypic selection," explained R. de Jonge and coauthors at Erasmus University. However, this method "can result in biased selection due to restriction or instability of the cloned DNA, or toxicity of the encoded products."
To remedy this, de Jonge and coworkers "created two mutant libraries in the human pathogen Helicobacter pylori using a simple, direct...
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