Published in TB and Outbreaks Week, August 5th, 2003
A study from Brazil "describes the cloning and characterization of a gene encoding a cysteine proteinase isoform, Llacys1, expressed in amastigote forms of Leishmania amazonensis. Recombinant clones containing the Llacys1 gene were isolated from genomic DNA by PCR amplification and screening of an amastigote cDNA library.
"Sequence analysis of the Llacys1 gene showed a high identity to sequence of Leishmania pifanoi Lpcys1, L. major cpa, L. mexicana LCPa, and L. chagasi Ldccys2. The Llacys1 gene is present...
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