Infectious Disease
Rapid PCR assay cannot replace PFGE in epidemiological MRSA research
Published in TB and Outbreaks Week, September 9th, 2003
"Pulsed-field gel electrophoresis (PFGE) is considered the ''gold standard'' for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA)," scientists in Switzerland explained. "However, the method is time-consuming and expensive, and its discriminatory power may not be necessary in outbreak situations."
In their study, A. Stranden and coauthors at the University of Basel Hospital "used a rapid multiplex PCR-based method with published primers and compared the results with those obtained by PFGE."
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