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Tuberculosis Diagnostics

Resazurin microtiter assay cheap, rapid, and accurate for TB resistance testing

Published in TB and Outbreaks Week, December 23rd, 2003

The colorimetric resazurin microtiter assay is a cheap, rapid, and accurate test for mycobacterial drug resistance.

According to recent research from Belgium, "The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests."

A. Martin and coauthors at the Institute of Tropical Medicine in Antwerp "tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method."

"By visual reading, MICs were obtained after eight days," they noted....

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