SARS
Real-time PCR enables rapid diagnosis
Published in TB and Outbreaks Week, February 10th, 2004
In a recent study from China, "An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR."
"Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays," explained L.T. Lau and coauthors at Peking University. "An amplicon of the size...
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