Infectious Disease

Panfungal PCR useful for cerebral aspergillosis diagnosis

Published in TB and Outbreaks Week, March 2nd, 2004

Panfungal PCR can facilitate cerebral aspergillosis diagnosis.

Physicians in Japan described their experience treating a "patient with osteosarcoma in whom a brain abscess developed after autologous peripheral stem-cell transplantation."

"Serologic markers of fungal infection were negative, but fungal DNA was detected in the cerebrospinal fluid (CSF) by panfungal polymerase chain reaction (PCR) assay using primers derived from fungal 18S ribosomal RNA (rRNA) gene," according to H. Komatsu and coauthors at the National Defense Medical College in Saitama. "The sequence of PCR products on the panfungal assay was identical to the 18S rRNA genes of...

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Source: TB and Outbreaks Week (2004-03-02)

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