Tuberculosis Drug Resistance
Multiplex PCR amplimer conformation detects gyrA mutations
Published in TB and Outbreaks Week, March 23rd, 2004
According to recent research from China, a "new strategy known as multiplex PCR amplimer conformation (MPAC) was developed for detection of mutation in the gyrA gene of 138 clinical isolates of Mycobacterium tuberculosis."
"The method generated a single-stranded and heteroduplex DNA banding pattern of multiplex PCR amplimers of the region of interest that was extremely sensitive to specific mutations, thus enabling much more sensitive and reliable mutation analysis compared to the standard single-stranded conformation...
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