West Nile Virus
SYBR green-based assay detects 100% of West Nile virus target region variants
Published in TB and Outbreaks Week, June 29th, 2004
According to published research from the United States, "Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus (WNV). Rapidly evolving RNA viruses present a challenge for diagnosis because they accumulate mutations that may render them undetectable."
"To explore the effect of sequence variations on assay performance, we generated every possible single point mutation within the target region of the widely used TaqMan assay for WNV and found that the TaqMan assay failed to detect 47% of possible...
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